Quiz #5 Study Tips

Here are some topics to review for next weeks QUIZ:

1. Mitosis & Meiosis

• Chromosomes, be able to label all parts: sister chromatids, centromere, homologous chromosomes, tetrads, etc.
• Understand the steps and be able to recognize and draw
• PMAT and cell cycle
• Compare and contrast the two processes
• What is meant by haploid and diploid chromosome number and the number in humans
• Plant vs. Animal cells

2. Karyotypes

• What they are and used for
• Recognize different syndromes discussed by chromosome
• Down's, Turner's and Klinefelter's syndrome

3. Protein Synthesis

• DNA vs. RNA
• mRNA, tRNA and DNA sequences
• Transcription
• Translation
• Start and Stop codons

Websites for info on cell division, genetics, karyotypes and Protein synthesis

General info:

http://www.biology.arizona.edu/cell_bio/cell_bio.html

http://www.cellsalive.com/mitosis.htm

Whitefish blastula mitosis:

http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/cell_division/wf_review.html

http://www.uoguelph.ca/zoology/devobio/210labs/mitosis1.html

Onion Root tip mitosis:

http://biog-101-104.bio.cornell.edu/BioG101_104/tutorials/cell_division/onion_review.html

http://www.biology.arizona.edu/cell_bio/activities/cell_cycle/cell_cycle.html

http://micro.magnet.fsu.edu/micro/gallery/mitosis/mitosis.html

http://biology.nebrwesleyan.edu/benham/mitosis/

Karyotypes:

http://gslc.genetics.utah.edu/units/disorders/karyotype/

http://www.biology.arizona.edu/human_bio/activities/karyotyping/karyotyping.html

http://www.biology.arizona.edu/human_bio/current/new_karyotyping/new_karyotyping.html

Protein synthesis simulation:

http://www.wisc-online.com/objects/index_tj.asp?objid=AP1302

http://learn.genetics.utah.edu/units/basics/transcribe/

Gen. Bio tutorials

http://www.sumanasinc.com/webcontent/anisamples/biology/biology.html

Thanks to Karen for all these links.

Quiz #4- Respiration Quiz tips

Quiz 4- Respiration lab TIPS

Know everything about following from your lab manual

• Aerobic respiration
• Anaerobic respiration, fermentation
• Differences between both of them
• Glycolysis & Krebs cycle and where they take place
• How much energy is produced
• The succinate to fumarate reaction
• Role of DCPIP, what gets reduced and what gets oxidized, the color of DCPIP during this change.
• What were the settings on spec for measuring change in color of DCPIP and why
• Role of yeast in break down of glucose
• Find out which sugars and monosaccharides and which are disaccharides
• Can yeast breakdown disaccharides and role of Lactaid.

LAB COATS + exam 1 grades

Hi guys,

Please do not forget to bring in your lab coats. If you forget we do not have any option but to send you back to get it.

Also your exam 1 grades are posted. Click on the link below and on that webpage go all the way to bottom to find Dr. Abbotts lecture and exam 1 grades.

Dr. Abbott's stuff

Quiz 3 Study Tips

Understand the following concepts:

• Diffusion - diffusion rates and the things that affect
• Osmosis and Tonicity - hypotonic, isotonic, hypertonic and the effects the on cells, both animal and plant
• Dialysis - how it differs from osmosis
• Eukaryotes vs. Prokaryotes
• Basic organelles and functions
• Bacteria: Gram + vs. Gram -, shape

Experiments:

1. Diffusion : calculations, materials
2. Dialysis: materials, results and what they indicate, all reagents and how they work
3. Microscopy: basic parts, objectives, total magnification, field diameter (how to find and calculate), conversion mili- to micro-, techniques, drawings
4. Cell Types: names of cell types and organisms, defining characteristics of cell types and organisms

QUIZ 1 Answer Key

Section 15 Quiz average=5.6/7
1. a.) Graph, if you have questions see TA during office hours
b.) 0.6-0.7 were acceptable answers
2. 2 mL
3. Protein/BSA were acceptable answers for space #1, 2, and 4. Purple was the answer for space #3, and blue was the answer for space #5.
4. Lambda max
5. a.) 3600mm b.) 4,200,000 uL
6. qualitative- observation, characteristic ex. color
quantitative-actual measurable value ex. mass, volume

Section 16 Quiz average=5.8/7
1. a.) dependent variable - absorbance at 620nm; independent variable - concentration mg/mL
b.) 1 mg/mL
2. TUBE #1: 7.5 mg, 0.75 mg/mL
TUBE #2: 12.5 mg, 1.25 mg/mL
3. Same as Section15 (look above)
4. Same as Section15 (look above)
5. 0.0554 mL
6. 1.5x10^6

Enzyme lab report: trouble shooting

Hi guys,

Just a quick reminder that enzyme lab report is due on February 13th. All the queries that you post to us about how to write and what to write will be shared here.

How to write references
There are numerous ways to cite something but lets all stick to an example that I am giving here.

For a sentence, "Enzymes are highly specific for the reaction they catalyze and they are typically grouped according to their specific function. They also work by lowering the activation energy".
An ideal way to cite this would be, "Enzymes are highly specific for the reaction they catalyze and they are typically grouped according to their specific function1. They also work by lowering the activation energy2".
Please super script 1 and 2. Some how it is not supported on this blog

At the end of your lab report you will mention.
1 Lombard, K. and Terry, T. Analysis of amino acids and proteins. Biology 107 Laboratory manual. Spring 2007 ed., 69.
2 Campbell,N.A. and Reece,J.B. An introduction to metabolism. Biology, 7th ed.,152.

The way above format works:
1 Name of authors, Name of chapter, Book, Year, Page nos.

Quiz #2-Enzymes Quiz tips

Remember to go through

☞Theory of enzymes: what are they, their function, enzyme catalyzed reactions and how it happens.

☞Role of inhibitor in inhibiting reactions

☞What all reagents/chemicals were used for the enzymatic reaction that we performed and their significance/use/function. i.e. What enzyme, substrate, inhibitor and so on…..

☞Different kind of graphs or curves that you got for the factors that affect enzyme reaction.

☞General calculation and different controls used and why.
Good luck.